5SZQ

Protocadherin Gamma A4 extracellular cadherin domains 3-6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-29
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97919
Spacegroup name	P 21 21 21
Unit cell lengths	31.890, 63.640, 344.310
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.957 - 2.608
R-factor	0.2522
Rwork	0.251
R-free	0.28200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	EC3-4 of 5SZM EC5 of 5SZN and EC6 of 5SZR
RMSD bond length	0.004
RMSD bond angle	0.658
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	172.160	2.670
High resolution limit [Å]	2.560	2.560
Rmerge	0.112	3.118
Number of reflections	23763
<I/σ(I)>	6.6
Completeness [%]	99.3	97.5
Redundancy	4.1	3.3
CC(1/2)	0.998	0.434

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8.5	295	0.1 M ammonium sulfate, 9% (w/v) PEG20000, 18% PEG550MME, 0.1 M Morpheus Buffer System 3 (Tris/Bicine; Molecular Dimensions) pH 8.5