5SZP

Protocadherin Gamma B7 extracellular cadherin domains 1-4 P21 crystal form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-07-22
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97919
Spacegroup name	P 1 21 1
Unit cell lengths	83.813, 45.549, 127.072
Unit cell angles	90.00, 96.91, 90.00

Refinement procedure

Resolution	19.977 - 3.100
R-factor	0.2586
Rwork	0.256
R-free	0.30980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5szo
RMSD bond length	0.002
RMSD bond angle	0.585
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.950	3.310
High resolution limit [Å]	3.100	3.100
Rmerge	0.180	1.512
Number of reflections	17677
<I/σ(I)>	5.5	0.8
Completeness [%]	99.5	99.2
Redundancy	3.4	3.3
CC(1/2)	0.982	0.585

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	8.5	295	0.1 M Tris-Cl pH 8.5, 0.2 trimethylamine N-oxide, 5% (v/v) Jeffamine M-600 pH 7.0, 17% (w/v) PEG2000MME