5SZJ

Structure of human Rab10 in complex with the bMERB domain of Mical-cL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-05-01
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 21 21 2
Unit cell lengths	153.680, 61.870, 55.570
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.190 - 2.660
R-factor	0.2264
Rwork	0.224
R-free	0.26640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4lhw 5szg
RMSD bond length	0.004
RMSD bond angle	0.756
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.190	2.700
High resolution limit [Å]	2.660	2.660
Rmerge	0.137	1.590
Number of reflections	15861
<I/σ(I)>	12.06	1.35
Completeness [%]	100.0	100
Redundancy	11.8
CC(1/2)		0.591

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	18%-22% PEG 3350 0.2-0.3 M Sodium Acetate