5SZ8
Truncated hemolysin A Q125A/Y134A from P. mirabilis at 1.8 Angstroms resolution crystallized in a high salt condition
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-26 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.231, 69.231, 234.862 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.779 - 1.830 |
R-factor | 0.179 |
Rwork | 0.178 |
R-free | 0.20570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4w8q |
RMSD bond length | 0.017 |
RMSD bond angle | 1.479 |
Data reduction software | HKL-3000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.860 |
High resolution limit [Å] | 1.830 | 4.970 | 1.830 |
Rmerge | 0.085 | 0.045 | 0.803 |
Number of reflections | 51639 | ||
<I/σ(I)> | 9.1 | ||
Completeness [%] | 99.9 | 97.7 | 100 |
Redundancy | 22 | 24.4 | 22.1 |
CC(1/2) | 0.999 | 0.964 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 1.0 M Lithium sulfate monohydrate, 2% w/v Polyethylene glycol 8,000 |