Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-02-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 100.770, 113.450, 174.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.650 |
| R-factor | 0.1419 |
| Rwork | 0.140 |
| R-free | 0.17060 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1MWV |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.482 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0151) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.140 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.403 | |
| Total number of observations | 121936 | |
| Number of reflections | 228125 | |
| <I/σ(I)> | 8.8 | 1.8 |
| Completeness [%] | 95.2 | 92.4 |
| Redundancy | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 293 | 16-20% PEG 4000, 20% MPD, 0.1 M sodium citrate pH 5.6 |
