5SWI
Crystal structure of SpGH92 in complex with mannose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL7-1 |
Synchrotron site | SSRL |
Beamline | BL7-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-11-05 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.97531 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 130.003, 161.711, 208.404 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 127.760 - 2.150 |
R-factor | 0.198 |
Rwork | 0.197 |
R-free | 0.22120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wvy |
RMSD bond length | 0.009 |
RMSD bond angle | 1.225 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 127.760 |
High resolution limit [Å] | 2.150 |
Rmerge | 0.103 |
Number of reflections | 237386 |
<I/σ(I)> | 14.4 |
Completeness [%] | 99.9 |
Redundancy | 7.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 291 | 2.25 M (NH4)2SO4, 0.1M Bis-Tris |