5SVZ
HIV-1 Tat NLS in complex with importin alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-10 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9537 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 79.000, 89.831, 99.460 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.662 - 2.000 |
R-factor | 0.1752 |
Rwork | 0.174 |
R-free | 0.19240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5fc8 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.653 |
Data reduction software | iMOSFLM |
Data scaling software | Aimless (0.5.21) |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.662 | 29.660 | 2.050 |
High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
Rmerge | 0.095 | 0.028 | 1.057 |
Number of reflections | 46564 | ||
<I/σ(I)> | 13.4 | ||
Completeness [%] | 96.5 | 88.6 | 98.2 |
Redundancy | 4.6 | 4.3 | 4.6 |
CC(1/2) | 0.998 | 0.999 | 0.650 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 296 | 1.25 M sodium citrate pH 7 and 10 mM DTT |