5SVX

MORC3 CW in complex with histone H3K4me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-04
Detector	DECTRIS PILATUS3 R CdTe 300K
Wavelength(s)	1.28
Spacegroup name	P 21 21 2
Unit cell lengths	31.250, 68.550, 26.530
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.275 - 1.560
R-factor	0.1446
Rwork	0.143
R-free	0.17800
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	1.009
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.8.4_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.320	1.600
High resolution limit [Å]	1.560	1.560
Rmerge		0.180
Number of reflections	15728
<I/σ(I)>	24.3
Completeness [%]	100.0	99.7
Redundancy	17.6	15

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	2.5 M (NH4)SO4, 0.1 M sodium acetate