5SVW

Light-state Structure of Arabidopsis Thaliana Zeitlupe

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	80
Detector technology	CCD
Collection date	2014-03-06
Detector	ADSC QUANTUM 270
Wavelength(s)	0.9773
Spacegroup name	P 31 2 1
Unit cell lengths	86.221, 86.221, 200.478
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	41.614 - 2.290
R-factor	0.1691
Rwork	0.166
R-free	0.22780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3d72
RMSD bond length	0.016
RMSD bond angle	1.645
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.330
High resolution limit [Å]	2.290	2.290
Rmerge	0.063	0.220
Number of reflections	34852
<I/σ(I)>	27.6	5
Completeness [%]	87.0	82.8
Redundancy	2.6	2.4
CC(1/2)		0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M Tris ph 8.5, 0.2 M Sodium Acetate trihydrate, 30% w/v PEG 4K