5SVV
Structure and kinetics of the LOV domain of ZEITLUPE determine its circadian function in Arabidopsis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2014-03-06 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.979 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 85.427, 85.427, 198.775 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.367 - 2.101 |
R-factor | 0.1649 |
Rwork | 0.163 |
R-free | 0.20020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3d72 |
RMSD bond length | 0.012 |
RMSD bond angle | 1.464 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.251 | |
Number of reflections | 49510 | |
<I/σ(I)> | 36.6 | 12.8 |
Completeness [%] | 99.3 | 100 |
Redundancy | 7.9 | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1 M Tris ph 8.5, 0.2 M Sodium Acetate trihydrate, 30% w/v PEG 4K |