5SVQ
Crystal structure of the ATP-gated human P2X3 ion channel bound to competitive antagonist TNP-ATP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-11-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.979 |
Spacegroup name | H 3 2 |
Unit cell lengths | 120.450, 120.450, 235.990 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.819 - 3.250 |
R-factor | 0.2505 |
Rwork | 0.249 |
R-free | 0.28930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.004 |
RMSD bond angle | 0.649 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10_2142: ???)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 48.000 |
High resolution limit [Å] | 3.250 |
Number of reflections | 10651 |
<I/σ(I)> | 19.64 |
Completeness [%] | 99.8 |
Redundancy | 10.27 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 25% PEG 400, 100 mM MES, pH 6.85, 50 mM MgCl2 |