5SVJ

Crystal structure of the ATP-gated human P2X3 ion channel in the closed, apo state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	CCD
Collection date	2015-09-20
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0
Spacegroup name	H 3 2
Unit cell lengths	120.170, 120.170, 236.580
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.068 - 2.984
R-factor	0.2147
Rwork	0.212
R-free	0.25910
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.772
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2142: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.980
Number of reflections	13067
<I/σ(I)>	18.34
Completeness [%]	95.4
Redundancy	4.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	25% PEG 400, 100 mM MES, pH 6.85, 50 mM MgCl2