5SVF
IDH1 R132H in complex with IDH125
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2011-03-11 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 81.979, 154.993, 162.989 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.750 - 2.340 |
Rwork | 0.179 |
R-free | 0.22320 |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 162.990 |
High resolution limit [Å] | 2.340 |
Number of reflections | 88813 |
<I/σ(I)> | 17.7 |
Completeness [%] | 100.0 |
Redundancy | 6.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M Bis-Tris pH6.5, 1.45M tri-sodium citrate dihydrate |