5SVD
Nop9, a new PUF-like protein, prevents premature pre-rRNA cleavage to correctly process mature 18S rRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-14 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 67.501, 110.466, 114.846 |
| Unit cell angles | 90.00, 92.22, 90.00 |
Refinement procedure
| Resolution | 32.022 - 2.100 |
| R-factor | 0.1855 |
| Rwork | 0.185 |
| R-free | 0.22120 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.112 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX (1.9_1692) |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 96076 | |
| <I/σ(I)> | 12.5 | |
| Completeness [%] | 97.5 | |
| Redundancy | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 295 | 18% (w/v) PEG 3350, 0.2 M ammonium citrate |
