5SV9
Structure of the SLC4 transporter Bor1p in an inward-facing conformation
EM specimen
| Vitrification | Yes |
| Instrument | HOMEMADE PLUNGER |
EM Data Collection
| Microscope | FEI TECNAI F20 |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2 |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 5.9 |
| Number of Particle | 87 |
| Software | SPARX/EMAN2,FREALIX,CTFFIND,Situs,NAMD |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
