5SV4
Anti-Ricin A-chain Single Domain Antibody A3C8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR-H |
Temperature [K] | 150 |
Detector technology | CCD |
Collection date | 2014-10-03 |
Detector | BRUKER SMART 6000 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.883, 44.395, 143.770 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.920 - 2.700 |
R-factor | 0.20056 |
Rwork | 0.198 |
R-free | 0.24595 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4fhb |
RMSD bond length | 0.010 |
RMSD bond angle | 1.482 |
Data reduction software | SAINT |
Data scaling software | SADABS |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.920 | 2.800 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.445 | |
Number of reflections | 6826 | |
<I/σ(I)> | 12.24 | 3.47 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11.52 | 8.15 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 290 | 0.1 M Citric acid pH 5.0, 1.6 M Ammonium Sulfate |