5SV0
Structure of the ExbB/ExbD complex from E. coli at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-06-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 137.166, 104.779, 149.364 |
| Unit cell angles | 90.00, 91.77, 90.00 |
Refinement procedure
| Resolution | 46.000 - 2.600 |
| R-factor | 0.2118 |
| Rwork | 0.211 |
| R-free | 0.25760 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | DENZO (HKL2000) |
| Data scaling software | SCALEPACK (HKL2000) |
| Phasing software | PHASER (PHENIX) |
| Refinement software | PHENIX ((1.10_2142: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.000 | 2.690 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.900 | |
| Number of reflections | 126321 | |
| <I/σ(I)> | 14.1 | 1.2 |
| Completeness [%] | 96.3 | 95.7 |
| Redundancy | 3.5 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 288 | 100 mM HEPES-NaOH, pH 7.0, 100 mM CaCl2, and 22% PEG MME 550 |
