5SUO
Crystal structure of N-glycan transport solute binding protein (NgtS) from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-002 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-03-20 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.54187 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 60.910, 40.200, 93.890 |
| Unit cell angles | 90.00, 98.77, 90.00 |
Refinement procedure
| Resolution | 92.790 - 2.100 |
| R-factor | 0.2136 |
| Rwork | 0.211 |
| R-free | 0.26110 |
| Structure solution method | SAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.418 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 92.790 |
| High resolution limit [Å] | 1.730 |
| Number of reflections | 25262 |
| <I/σ(I)> | 25.4 |
| Completeness [%] | 99.9 |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 29 % PEG 1500, 11 % 2-methyl-2,4-pentanediol, 0.1 M Tris |
