5SUL
Inhibited state structure of yGsy2p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2014-11-06 |
Detector | DECTRIS PILATUS 300K |
Wavelength(s) | 0.919 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 122.447, 122.447, 279.361 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.040 - 3.300 |
R-factor | 0.16119 |
Rwork | 0.158 |
R-free | 0.22298 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3naz |
RMSD bond length | 0.011 |
RMSD bond angle | 1.665 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.360 |
High resolution limit [Å] | 3.300 | 3.300 |
Rmerge | 0.134 | 0.621 |
Number of reflections | 35194 | |
<I/σ(I)> | 7.59 | 2.13 |
Completeness [%] | 99.4 | 99.5 |
Redundancy | 4.8 | 5 |
CC(1/2) | 0.703 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | Peg 6000, Magnesium Chloride, Tris-HCl |