5SUK
G6P bound activated state of yeast glycogen synthase 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-06-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.979 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 191.984, 204.562, 205.819 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 145.090 - 2.880 |
| R-factor | 0.22098 |
| Rwork | 0.219 |
| R-free | 0.26004 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nb0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.132 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 145.090 | 2.950 |
| High resolution limit [Å] | 2.880 | 2.900 |
| Rmerge | 0.063 | 0.556 |
| Number of reflections | 84895 | |
| <I/σ(I)> | 17.95 | 2.51 |
| Completeness [%] | 98.0 | |
| Redundancy | 5 | 4.7 |
| CC(1/2) | 0.870 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Peg 300, Bis-Tris pH 6.5 |
