5SUJ

Crystal structure of uncharacterized protein LPG2148 from Legionella pneumophila

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2013-12-13
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97915
Spacegroup name	P 21 21 2
Unit cell lengths	122.884, 135.889, 64.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.573 - 2.356
R-factor	0.1809
Rwork	0.179
R-free	0.22250
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.912
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	HKL-3000
Refinement software	PHENIX (dev_2386)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.390
High resolution limit [Å]	2.350	6.370	2.350
Rmerge	0.082	0.026	0.778
Rmeas	0.087	0.028	0.852
Rpim	0.030	0.010	0.336
Total number of observations	363605
Number of reflections	44965
<I/σ(I)>	8.5
Completeness [%]	99.7	99.8	97.4
Redundancy	8.1	7.8	5.5
CC(1/2)		0.999	0.836

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M Sodium formate, 20% PEG3350, 4% Sucrose