5SUI
Ribosome assembly factor NSA1: C-terminal truncation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-02-14 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.327, 81.052, 87.371 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 59.420 - 1.300 |
| R-factor | 0.1296 |
| Rwork | 0.129 |
| R-free | 0.16180 |
| Structure solution method | SAD |
| RMSD bond length | 0.030 |
| RMSD bond angle | 2.429 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 59.420 |
| High resolution limit [Å] | 1.300 |
| Number of reflections | 93271 |
| <I/σ(I)> | 29.6 |
| Completeness [%] | 99.9 |
| Redundancy | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20.1%(v/v) PEG 1500, 13.4%(v/v) PEG 400, 0.1M HEPES/ Sodium hydroxide pH 7.5 |
