5SSX
Crystal Structure human formylglycine generating enzyme E130D mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-11 |
Detector | PSI PILATUS 6M |
Wavelength(s) | 0.999980 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 43.494, 61.640, 109.579 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.950 - 1.020 |
R-factor | 0.1238 |
Rwork | 0.123 |
R-free | 0.13750 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1z70 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 40.950 | 40.950 | 1.060 |
High resolution limit [Å] | 1.020 | 4.610 | 1.020 |
Rmerge | 0.052 | 0.030 | 0.842 |
Rmeas | 0.057 | 0.033 | 1.087 |
Total number of observations | 779553 | ||
Number of reflections | 142078 | 1844 | 7890 |
<I/σ(I)> | 14.49 | 42.4 | 0.89 |
Completeness [%] | 97.3 | 99.4 | 73.8 |
Redundancy | 5.487 | 6.048 | 1.871 |
CC(1/2) | 0.999 | 0.999 | 0.526 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 9.6 mg/mL protein in 20mM Tris/HCl pH8.0 mixed 1:1 with reservoir 0.1M Tris/HCl pH 8.5, 20-25% PEG4000, 0.2-0.3M CaCl2 |