5SKV

Crystal Structure of human phosphodiesterase 10 in complex with 6-cyclopropyl-N-[3-[(2-hydroxy-2-methylpropyl)carbamoyl]-1-methylpyrazol-4-yl]-3-(pyrimidin-5-ylamino)pyridine-2-carboxamide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-20
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.999900
Spacegroup name	H 3
Unit cell lengths	135.283, 135.283, 234.745
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	43.510 - 2.600
R-factor	0.2015
Rwork	0.199
R-free	0.25500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	inhouse model
RMSD bond length	0.005
RMSD bond angle	1.366
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.510	43.510	2.670
High resolution limit [Å]	2.600	11.630	2.600
Rmerge	0.268	0.105	1.509
Rmeas	0.288	0.114	1.621
Total number of observations	192154
Number of reflections	25709	325	1866
<I/σ(I)>	6.59	13.38	1.55
Completeness [%]	100.0	97.6	100
Redundancy	7.474	6.345	7.551
CC(1/2)	0.983	0.990	0.404

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	5-20 mg/mL protein in 25mM HEPES/NaOH pH7.5, 150mM NaCl, 50mM BME mixed 1:1 with reservoir 0.1M HEPES/NaOH pH7.5, 30% PEG550MME, 50mM MgCl2