5SHK

Crystal Structure of human phosphodiesterase 10 in complex with N-(6-methylpyridin-2-yl)-5-pyridin-3-yl-1,7-naphthyridin-8-amine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-04-14
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.000000
Spacegroup name	H 3
Unit cell lengths	134.736, 134.736, 235.207
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.840 - 2.000
R-factor	0.1901
Rwork	0.189
R-free	0.21810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	inhouse model
RMSD bond length	0.006
RMSD bond angle	1.305
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	34.840	34.840	2.050
High resolution limit [Å]	2.000	8.940	2.000
Rmerge	0.067	0.024	1.070
Rmeas	0.078	0.029	1.253
Total number of observations	409868
Number of reflections	107256	1097	7987
<I/σ(I)>	12.58	42.06	1.25
Completeness [%]	99.7	90.7	99.8
Redundancy	3.821	3.521	3.709
CC(1/2)	0.998	0.998	0.432

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	295	5-20 mg/mL protein in 25mM HEPES/NaOH pH7.5, 150mM NaCl, 50mM BME mixed 1:1 with reservoir 0.1M HEPES/NaOH pH7.5, 30% PEG550MME, 50mM MgCl2