5RHN
HISTIDINE TRIAD NUCLEOTIDE-BINDING PROTEIN (HINT) FROM RABBIT COMPLEXED WITH 8-BR-AMP
Experimental procedure
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | IMAGE PLATE |
Collection date | 1996-07 |
Detector | RIGAKU RAXIS II |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 40.320, 40.320, 143.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.310 |
R-factor | 0.162 |
Rwork | 0.162 |
R-free | 0.26400 |
RMSD bond length | 0.011 |
RMSD bond angle | 25.300 * |
Data reduction software | R-AXIS |
Data scaling software | R-AXIS |
Phasing software | X-PLOR |
Refinement software | X-PLOR |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.130 | 2.500 |
High resolution limit [Å] | 2.310 | 2.310 |
Rmerge | 0.084 | |
Total number of observations | 45935 * | |
Number of reflections | 5578 | |
<I/σ(I)> | 12 | |
Completeness [%] | 98.0 | 97.3 |
Redundancy | 8.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.4 * | 30% POLYETHYLENE GLYCOL 8000, 0.1 M SODIUM ACETATE, 0.1 M SODIUM CACODYLATE, PH 6.5 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | PEG8000 | 30 (%) | |
3 | 1 | reservoir | sodium acetate | 0.1 (M) | |
4 | 1 | reservoir | sodium cacodylate | 0.1 (M) | |
5 | 1 | drop | 8-Br-AMP | 0.2 (mM) | |
6 | 1 | drop | 150 (mM) | ||
7 | 1 | drop | Tris-Cl | 20 (mM) | pH7.4 |