5RG5
Crystal Structure of Kemp Eliminase HG3.3b in unbound state, 277K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.3.1 |
Synchrotron site | ALS |
Beamline | 8.3.1 |
Temperature [K] | 277 |
Detector technology | PIXEL |
Collection date | 2018-06-01 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.1158 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.230, 80.030, 98.910 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 37.090 - 1.620 |
Rwork | 0.142 |
R-free | 0.16650 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 37.090 | 37.100 | 1.650 |
High resolution limit [Å] | 1.620 | 4.400 | 1.620 |
Rmeas | 0.169 | 0.070 | 1.368 |
Rpim | 0.047 | 0.020 | 0.407 |
Total number of observations | 998006 | 49952 | 41801 |
Number of reflections | 77569 | 4168 | 3834 |
<I/σ(I)> | 8.9 | 27.2 | 1 |
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 12.9 | 12 | 10.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277 | 1.8-2.4 M Ammonium Sulfate, 4mg/mL Protein Concentration, 100mM Sodium Acetate |