5RAT
Effects of temperature on protein structure and dynamics: X-ray crystallographic studies of the protein ribonuclease-A at nine different temperatures from 98 TO 320 K
Experimental procedure
| Temperature [K] | 220 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 30.140, 38.230, 53.160 |
| Unit cell angles | 90.00, 106.96, 90.00 |
Refinement procedure
| Resolution | ? - 1.500 |
| R-factor | 0.151 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 0.036 |
| Refinement software | PROLSQ |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.500 * |
| Number of reflections | 10235 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 5.5 * | 20 * | taken from Gilbert, W.A. et al. (1982) J. Raman. Spectrosc., 12, 173-179. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 12 (mg/ml) | |
| 2 | 1 | 1 | 0.2 (M) | ||
| 3 | 1 | 1 | methanol | 50 (%(v/v)) |
