5R68
XChem group deposition -- Crystal Structure of human YEATS4 in complex with FM000199e
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04-1 |
| Synchrotron site | Diamond |
| Beamline | I04-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.91587 |
| Spacegroup name | P 1 |
| Unit cell lengths | 44.880, 46.810, 58.340 |
| Unit cell angles | 67.39, 68.46, 69.57 |
Refinement procedure
| Resolution | 41.960 - 1.640 |
| R-factor | 0.2212 |
| Rwork | 0.220 |
| R-free | 0.25120 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 5vna |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.680 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 41.930 | 41.930 | 1.680 |
| High resolution limit [Å] | 1.640 | 7.330 | 1.640 |
| Rmerge | 0.080 | 0.032 | 0.670 |
| Rmeas | 0.113 | 0.045 | 0.948 |
| Rpim | 0.080 | 0.032 | 0.670 |
| Total number of observations | 82967 | 1029 | 6115 |
| Number of reflections | 46555 | ||
| <I/σ(I)> | 8.5 | 27.5 | 1.1 |
| Completeness [%] | 96.1 | 97.2 | 95.2 |
| Redundancy | 1.8 | 2 | 1.8 |
| CC(1/2) | 0.980 | 0.993 | 0.614 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.1M tris pH 7.5, 0.15M lithium sulfate, 40% PEG400 |
