5QUU

HumRadA1 as soaking control with 10% DMSO

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2010-02-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9535
Spacegroup name	P 21 21 21
Unit cell lengths	40.090, 61.722, 86.525
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.263 - 1.248
R-factor	0.1566
Rwork	0.156
R-free	0.17450
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.263	43.263	1.320
High resolution limit [Å]	1.250	3.730	1.250
Rmerge	0.064	0.033	0.422
Rmeas	0.069	0.036	0.466
Total number of observations	396716
Number of reflections	59575	2169	9129
<I/σ(I)>	18.12	42.16	4.11
Completeness [%]	98.5	87.8	94.8
Redundancy	6.659	5.817	5.333
CC(1/2)	0.999	0.998	0.892

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	50 mM Na/K phospate, 5% PEG1000H