5QUM
Structure of unliganded HumRadA1.4
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2009-04-02 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.514 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 67.819, 69.501, 81.204 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.750 - 1.930 |
R-factor | 0.2352 |
Rwork | 0.232 |
R-free | 0.28910 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 34.750 | 34.750 | 2.040 |
High resolution limit [Å] | 1.930 | 5.730 | 1.930 |
Rmerge | 0.082 | 0.024 | 1.927 |
Rmeas | 0.089 | 0.026 | 2.108 |
Total number of observations | 203005 | ||
Number of reflections | 29549 | 1229 | 4543 |
<I/σ(I)> | 14.68 | 36.89 | 0.94 |
Completeness [%] | 99.3 | 99.2 | 96 |
Redundancy | 6.87 | 6.669 | 5.972 |
CC(1/2) | 0.999 | 1.000 | 0.638 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 50 mM Na/K phospate, 5% PEG1000H |