5QUL
Structure of unliganded HumRadA1.3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.572, 73.055, 69.218 |
| Unit cell angles | 90.00, 89.95, 90.00 |
Refinement procedure
| Resolution | 36.528 - 1.278 |
| R-factor | 0.1985 |
| Rwork | 0.197 |
| R-free | 0.23080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 36.528 | 36.528 | 1.360 |
| High resolution limit [Å] | 1.280 | 3.820 | 1.280 |
| Rmerge | 0.071 | 0.029 | 0.871 |
| Rmeas | 0.083 | 0.034 | 1.029 |
| Total number of observations | 371035 | ||
| Number of reflections | 102758 | 3869 | 16515 |
| <I/σ(I)> | 10.53 | 36.67 | 1.48 |
| Completeness [%] | 98.6 | 96.6 | 98.3 |
| Redundancy | 3.611 | 3.572 | 3.514 |
| CC(1/2) | 0.998 | 0.998 | 0.632 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 50 mM Na/K phospate, 5% PEG1000H |
