5QUK

Structure of unliganded HumRadA1.2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2009-03-08
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9795
Spacegroup name	P 21 21 2
Unit cell lengths	69.045, 73.027, 40.473
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.514 - 1.160
R-factor	0.179
Rwork	0.178
R-free	0.19650
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.514	36.514	1.230
High resolution limit [Å]	1.160	3.460	1.160
Rmerge	0.059	0.021	1.091
Rmeas	0.064	0.023	1.196
Total number of observations	494912
Number of reflections	71657	2874	11240
<I/σ(I)>	19.03	68.08	1.57
Completeness [%]	99.6	98.5	97.8
Redundancy	6.907	6.615	5.942
CC(1/2)	1.000	1.000	0.646

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	50 mM Na/K phospate, 5% PEG1000H