5QUJ

HumRadA1.2 soaked with napht-2,3-diol overnight 10 mM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	CCD
Collection date	2009-02-16
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9794
Spacegroup name	P 21 21 2
Unit cell lengths	69.150, 72.939, 40.378
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.378 - 1.420
R-factor	0.1713
Rwork	0.170
R-free	0.20330
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.378	40.378	1.500
High resolution limit [Å]	1.420	4.230	1.420
Rmerge	0.114	0.042	1.380
Rmeas	0.123	0.046	1.515
Total number of observations	275344
Number of reflections	39374	1636	6188
<I/σ(I)>	11.55	36.05	1.41
Completeness [%]	99.7	99.8	98.3
Redundancy	6.993	6.677	5.832
CC(1/2)	0.998	0.998	0.506

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	291	50 mM Na/K phospate, 5% PEG1000H