5QT4
T. brucei FPPS in complex with CID 3599333
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-16 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.000000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 61.077, 61.077, 344.223 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 52.894 - 2.324 |
R-factor | 0.283 |
Rwork | 0.281 |
R-free | 0.32460 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdbid 4ryp |
Data reduction software | XDS (20180808) |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.894 | 2.595 |
High resolution limit [Å] | 2.324 | 2.324 |
Rmerge | 0.073 | 1.862 |
Rmeas | 0.120 | 16.004 |
Rpim | 0.028 | 3.575 |
Total number of observations | 255141 | 42821 |
Number of reflections | 9284 | 2192 |
<I/σ(I)> | 26.4 | 0.3 |
Completeness [%] | 52.8 | 9.7 |
Redundancy | 17 | 16.4 |
CC(1/2) | 0.999 | 0.375 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 0.12 M CsCl 12 % w/v PEG 3350, 12 % v/v DMSO |