Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-10 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 1 |
| Unit cell lengths | 37.020, 52.970, 58.580 |
| Unit cell angles | 70.75, 71.79, 73.21 |
Refinement procedure
| Resolution | 42.100 - 2.000 |
| R-factor | 0.155 |
| Rwork | 0.152 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.972 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.250 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.068 | 0.195 |
| Number of reflections | 23183 | |
| <I/σ(I)> | 16 | 5.1 |
| Completeness [%] | 94.2 | 84.6 |
| Redundancy | 3.8 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 293 | 100MM SODIUM ACETATE PH 4.5, 200MM AMMONIUM ACETATE, 25% PEG 8000. PROTEIN CONCENTRATION 7 MG/ML, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
