5Q10
Ligand binding to FARNESOID-X-RECEPTOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-19 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 |
Unit cell lengths | 93.957, 93.957, 48.020 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.680 - 2.200 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.24300 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 0.950 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.600 | 2.290 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.112 | 0.399 |
Number of reflections | 11238 | 648 |
<I/σ(I)> | 9.11 | 1.5 |
Completeness [%] | 90.3 | 46.8 |
Redundancy | 3.65 | 0.61 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | evaporation, hanging drop | 8.5 | 298 | 0.2M MgCl2, 0.1M Tris pH 8.5, 25%PEG3350 |