5Q0T
Ligand binding to FARNESOID-X-RECEPTOR
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-02-02 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 1.0 |
Spacegroup name | P 65 |
Unit cell lengths | 93.940, 93.940, 47.670 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 11.210 - 2.140 |
R-factor | 0.194 |
Rwork | 0.191 |
R-free | 0.25900 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.030 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.900 | 2.240 |
High resolution limit [Å] | 2.140 | 2.140 |
Rmerge | 0.051 | 0.478 |
Number of reflections | 10861 | 857 |
<I/σ(I)> | 17.04 | 0.97 |
Completeness [%] | 80.5 | 48.2 |
Redundancy | 3.97 | 0.68 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | evaporation, hanging drop | 7 | 298 | 3.5 M Sodium Formate pH 7.0 |