5Q0K
Ligand binding to FARNESOID-X-RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-07-18 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 65 |
| Unit cell lengths | 93.520, 93.520, 47.830 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 19.630 - 1.800 |
| R-factor | 0.213 |
| Rwork | 0.210 |
| R-free | 0.27100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.000 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.800 | 1.890 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.165 | 1.044 |
| Number of reflections | 22191 | 2869 |
| <I/σ(I)> | 9.34 | 1.26 |
| Completeness [%] | 99.6 | 97.8 |
| Redundancy | 11.04 | 9.87 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | evaporation, hanging drop | 7.5 | 298 | 0.2M MgCl2, 0.1M HEPES pH 7.5, 25%PEG3350 |
