5PZW
Human liver fructose-1,6-bisphosphatase 1 (fructose 1,6-bisphosphate 1-phosphatase, E.C.3.1.3.11) complexed with the allosteric inhibitor 1-(3-chlorophenyl)sulfonyl-3-[5-[(3-chlorophenyl)sulfonylcarbamoylamino]pentyl]urea
Experimental procedure
| 実験手法 | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-08-04 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 21 21 21 |
| 格子定数 [Å] | 67.204, 83.617, 278.817 |
| 格子定数 [度] | 90.00, 90.00, 90.00 |
精密化法
| 残基 | 29.946 - 2.000 |
| R因子 | 0.1848 |
| Rwork | 0.182 |
| R-free | 0.23550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| 結合長の平均二乗偏差(RMSD) [Å] | 0.007 |
| 結合角の平均二乗偏差(RMSD) [度] | 0.894 |
| Refinement software | PHENIX (1.11.1_2575) |
Quality characteristics
| Overall | |
| 分解能 [Å] (低) | 29.946 |
| 分解能 [Å] (高) | 2.000 |
| 独立反射数 | 106085 |
| 完全性 [%] | 99.0 |
結晶化条件
| 結晶ID | 方法 | pH | 温度 | 溶液条件 |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 300 | 0.1M HEPES pH 7.0, 0.1M AMMONIUM ACETATE, 12% PEG 3350 |
