5PRC

PHOTOSYNTHETIC REACTION CENTER FROM RHODOPSEUDOMONAS VIRIDIS (ATRAZINE COMPLEX)

Experimental procedure

Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	263
Detector technology	IMAGE PLATE
Collection date	1993-09
Detector	MARRESEARCH
Spacegroup name	P 43 21 2
Unit cell lengths	223.500, 223.500, 113.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.350
R-factor	0.192 *
Rwork	0.190
R-free	0.23400 *
Structure solution method	DIFFERENCE FOURIER / SA OMIT MAPS
Starting model (for MR)	6prc
RMSD bond length	0.011
RMSD bond angle	23.800 *
Data reduction software	MOSFLM
Data scaling software	CCP4 ((ROTAVATA)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000 *	2.360
High resolution limit [Å]	2.300	2.300
Rmerge	0.079	0.268 *
Total number of observations	252656 *
Number of reflections	92322 *
<I/σ(I)>	7.8	1.9
Completeness [%]	78.5 *	57.4 *
Redundancy	2.7	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6		pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	1.5 (M)
2	1	reservoir	ammonium sulfate	3 (M)	or 2.6M or 2.4M