5OYO
Crystal structure of BlaC from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-10-31 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.96773 |
| Spacegroup name | P 1 |
| Unit cell lengths | 39.589, 41.417, 76.523 |
| Unit cell angles | 104.29, 90.07, 90.67 |
Refinement procedure
| Resolution | 40.130 - 2.100 |
| R-factor | 0.20803 |
| Rwork | 0.206 |
| R-free | 0.25043 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gdn |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.658 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.130 | 2.000 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmerge | 0.090 | 0.586 |
| Rpim | 0.074 | 0.572 |
| Number of reflections | 33346 | 2851 |
| <I/σ(I)> | 6.8 | 1.3 |
| Completeness [%] | 94.2 | 81.4 |
| Redundancy | 2.2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M sodium acetate pH 5.0, 25% PEG1500 |
