5OYE
GH5 endo-xyloglucanase from Cellvibrio japonicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-05 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9795 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.343, 95.830, 157.208 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 81.830 - 1.900 |
R-factor | 0.2374 |
Rwork | 0.234 |
R-free | 0.29997 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5OYC.PDB |
RMSD bond length | 0.018 |
RMSD bond angle | 1.933 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | REFMAC |
Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.830 | 1.940 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.081 | |
Rpim | 0.031 | 0.592 |
Number of reflections | 66811 | 4194 |
<I/σ(I)> | 8.5 | |
Completeness [%] | 100.0 | 100 |
Redundancy | 7.7 | 7.6 |
CC(1/2) | 0.998 | 0.813 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 5.5 | 291 | ammonium sulphate, MES buffer |