5OXA
Structure of the S205A mutant of the Cyan Fluorescent Protein Cerulean at pH 7.0
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.977178 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 50.960, 63.320, 69.330 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.750 - 1.160 |
| R-factor | 0.11688 |
| Rwork | 0.116 |
| R-free | 0.13856 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2wso |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.689 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.760 | 1.190 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmeas | 0.036 | 0.885 |
| Number of reflections | 78153 | 5697 |
| <I/σ(I)> | 24.64 | 2.12 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 7.06 | 6.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100mM HEPES buffer pH 7.0, 12% PEG8000, 100mM MgCl2 |
