5OX7

Structure of P110 from Mycoplasma genitalium at 2.4A with potassium ion

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	78
Detector technology	PIXEL
Collection date	2016-11-25
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.97895
Spacegroup name	I 2 2 2
Unit cell lengths	109.603, 151.293, 176.245
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	62.540 - 2.400
R-factor	0.19363
Rwork	0.191
R-free	0.23971
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5MZB
RMSD bond length	0.024
RMSD bond angle	2.370
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.540	2.530
High resolution limit [Å]	2.400	2.400
Rmerge	0.049	0.738
Rmeas		0.877
Rpim		0.469
Number of reflections	56936
<I/σ(I)>	15.3	1.8
Completeness [%]	99.6	99.8
Redundancy	3.4	3.4
CC(1/2)		0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350 21% 0.2M Tri-potassium citrate