5OWV

An oligomerised bacterial dynamin pair provides a mechanism for the long-range sensing and tethering of membranes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-05-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97626
Spacegroup name	P 21 21 21
Unit cell lengths	112.590, 226.058, 317.892
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	106.130 - 3.720
R-factor	0.2606
Rwork	0.259
R-free	0.28640
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.858
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall
Low resolution limit [Å]	184.200
High resolution limit [Å]	3.720
Rmerge	0.089
Number of reflections	163788
<I/σ(I)>	11.1
Completeness [%]	99.7
Redundancy	4.9
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.7-1.0 M succinate pH 7.0