Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-05-01 |
Detector | MAR CCD 130 mm |
Wavelength(s) | 0.934 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 58.372, 126.534, 69.649 |
Unit cell angles | 90.00, 100.17, 90.00 |
Refinement procedure
Resolution | 28.723 - 2.000 |
R-factor | 0.1756 |
Rwork | 0.173 |
R-free | 0.21930 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.643 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.723 | |
High resolution limit [Å] | 2.000 | |
Rmerge | 0.110 | 0.502 |
Number of reflections | 66945 | 9691 |
<I/σ(I)> | 21.2 | 3.5 |
Completeness [%] | 99.8 | 99.1 |
Redundancy | 9 | 8.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 295 | 5 mg/ml protein in 5 mM Tris-HCl, pH 7.4 equilibrated against 90 mM (NH4)2SO4, 50 mM Na Cacodylate, pH 6.5, 13% PEG 8000 |