5OWQ

Human STK10 bound to dovitinib

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2012-02-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.9173
Spacegroup name	P 1 21 1
Unit cell lengths	57.570, 50.400, 114.570
Unit cell angles	90.00, 96.60, 90.00

Refinement procedure

Resolution	57.190 - 2.700
R-factor	0.26535
Rwork	0.263
R-free	0.30495
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	STK10
RMSD bond length	0.014
RMSD bond angle	1.693
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.190	2.860
High resolution limit [Å]	2.700	2.700
Rmerge	0.140	0.610
Number of reflections	16619	2672
<I/σ(I)>	4.7	1.3
Completeness [%]	91.4	91.8
Redundancy	2.8	2.7
CC(1/2)	0.984	0.692

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.1M sodium formate, 15%(w/v) PEG 3350