5OWN
Structure of TgPLP1 MACPF domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-01-30 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9686 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.230, 206.580, 82.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 55.800 - 3.110 |
| R-factor | 0.2363 |
| Rwork | 0.235 |
| R-free | 0.26170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ouo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.691 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 64.580 |
| High resolution limit [Å] | 3.110 |
| Number of reflections | 15082 |
| <I/σ(I)> | 12.9 |
| Completeness [%] | 100.0 |
| Redundancy | 31.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1M sodium citrate tribasic dihydrate, pH 5.5, 22% PEG 1000 |
