5OW4
Crystal structure of a protease-resistant fragment of the Trypanosoma cruzi gamete fusion protein HAP2 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06SA |
Synchrotron site | SLS |
Beamline | X06SA |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2015-03-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 150.090, 150.090, 123.780 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 49.130 - 3.100 |
R-factor | 0.2344 |
Rwork | 0.233 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5mf1 |
RMSD bond length | 0.008 |
RMSD bond angle | 0.990 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.310 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmeas | 0.201 | 2.493 |
Number of reflections | 15453 | 2389 |
<I/σ(I)> | 11.4 | 1.3 |
Completeness [%] | 97.7 | 98.2 |
Redundancy | 8.9 | 8.6 |
CC(1/2) | 0.997 | 0.410 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 100mM CHES pH9.0 200mM NaCl 10%w/v PEG 8K |